COMPARATIVE STUDIES ON CHROMATOGRAPHICALLY PURIFIED HISTONES
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چکیده
منابع مشابه
Properties of chromatographically purified bovine pancreatic deoxyribonuclease.
Preparations of bovine pancreatic deoxyribonuclease obtained by the ammonium sulfate precipitation procedure of Kunitz can be further purified by chromatography on sulfoethyl-Sephadex at pH 4.70. The instability of earlier preparations of the enzyme has usually been attributed to the sensitivity of the protein to proteolytic digestion. This hypothesis has been confirmed; treatment of the starti...
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1. The possibilities of fractionating histones and histone fractions by means of countercurrent distribution between two phases formed by water and butan-2-ol, in the presence of various concentrations of trichloroacetic acid, have been examined. 2. Although the principal histone fractions differ considerably in their partition ratios, a satisfactory resolution of the principal histone fraction...
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Bacteriophages offer interesting alternatives to antibodies for the specific capture and detection of pathogenic bacteria onto biosensing surfaces. Procedures for the optimal chemical immobilization of lytic bacteriophages onto surfaces are presented. More specifically, the removal of lysate contaminants from bacteriophage suspensions by size exclusion chromatography significantly increases the...
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Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contai...
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3-Hydroxyanthranilic acid oxidase is known to cat,alyze the oxidation of 3-hydroxyanthranilic acid to a very unstable aliphatic compound, which may either be spontaneously transformed into quinolinic acid or undergo enzymic conversion to picolinic or nicotinic acid, or both (l-3). According to Mason (4), this enzyme has been classified as an “oxygen transferase,” since the oxygen consumed, 1 mo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64938-5